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以后地位:首页> 新闻资讯> 新品公布 >Thermo(invitrogen)单线态氧荧光探针 Singlet Oxygen Sensor Green(SOSG)
Thermo(invitrogen)单线态氧荧光探针 Singlet Oxygen Sensor Green(SOSG)
>###nbsp;    作者:ag真人原创     文章泉源:ag真人生物    

单线态氧荧光探针 Singlet Oxygen Sensor Green(SOSG)



产品要害词:

Singlet Oxygen Sensor Green®, SOSG;单线态氧(Singlet Oxygen,1O2);单线态氧荧光探针;S36002;Thermo Fisher; ROS(Reactive oxygen species)活性氧物质;

 

活性氧惹起的很多心理毁伤都大概归因于单线态氧(1O2),包罗与脱氧鸟苷选择性反响构成8-羟基脱氧鸟苷(8-OHdG)的核酸修饰。单线态氧的寿命充足长(水中有4.4μs)使其可以充实分散进入细胞和构造。实行室中通常用三种之一的办法来天生单线态氧:1)利用感光染料对二氧(3O2)举行光化学反响来制备;2)经过对一种过氧化物或二氧环丁烷举行加热剖析制备;3)氧气流内经过微波放电来制备;单线态氧经过在1270nm处具弱化学发光的典范特性间接被检测。

 

Thermo fisher(molecular probe)公司开辟的Singlet Oxygen Sensor Green®, SOSG是现在贸易化可得且使用最普遍的单线态氧荧光探针,从质料学到药物学范畴,好比光动力学疗法。

 

【特异性强】

Singlet Oxygen Sensor Green®, SOSG高度选择性联合单线态氧(1O2)。不像其他的荧光和化学发光的单线态氧检测试剂,SOSG与其他活性氧物质(ROS),包罗羟基自在基(·OH)、超氧阴离子(·O2–)和一氧化氮(NO)无可见反响【见图1】。

图1、Singlet Oxygen Sensor Green (SOSG)的荧光反响和对单线态氧(1O2)的特异性。A)用荧光光度计(Ex/Em= 488/525nm)测定溶液内的荧光,溶液内含SOSG(1 µM )和亚甲基蓝(10 µM)溶于100mM pH 7.5 Tris Buffer; 单线态氧扫除剂叠氮钠(NaN3,1 mM);或50% D2O(重水,可以延伸1O2的寿命)。每20s完成一次荧光测定,每次测定距离30s(如灰条标示)。在这30s的距离中,样本表露于激光辐射(630–680 nm,<5 mW),招致亚甲基蓝光敏天生1O2。B)用荧光光度计(Ex/Em=488/525nm)测定溶液内的荧光,溶液为50mM pH 7 Tris buffer,1mM 黄嘌呤,SOSG(1 µM ),或含SOSG(1 µM ),或含二氢罗丹明123。约20s之后,参加50 mU/mL黄嘌呤氧化酶(XO)。XO催化黄嘌呤的氧化,发生尿酸和超氧化物。超氧化物刹时被H2O2降解。

 

【荧光特性】

Singlet Oxygen Sensor Green®, SOSG与单线态氧(1O2)反响前,探针最后发弱蓝色荧光,引发峰在372nm和393nm,发射峰在395nm和416nm。当含单线态氧(1O2),探针发射绿色荧光,光谱特性相似于荧光素(最大引发/发射波长约504/525nm)。

 

【线性化干系】

研讨发明Singlet Oxygen Sensor Green®, SOSG的荧光产品在某些溶液中随着工夫被降解,且在不含单线态氧的碱性pH溶液中,Singlet Oxygen Sensor Green®, SOSG会发荧光。不外,当举行得当的操纵,绿色荧光信号的强度与单线态氧(1O2)含量相干,且不会受其他ROS的分明搅扰。

 

【使用】

Singlet Oxygen Sensor Green®, SOSG证明能用来检测溶液和动物构造中的单线态氧(1O2)。

 

【利用办法】

1.       SOSG贮存液制备:收到货依照≤-20℃避光枯燥保管。用甲醇来制备贮存液,每100µg粉末参加33µl甲醇制备~5mM贮存液。正式实行前制备事情液,不克不及用完的事情液需抛弃,不行保管后再用。

2.       溶液检测:SOSG不具细胞膜浸透性,设计用来检测溶液情况内的单线态氧。最佳的浓缩缓冲液和事情浓度需依据履历来调解。发起肇始事情浓度为1-10µM。

 

【利用办法-来自文献】

一、文献泉源:Flors C et al. Imaging the production of singlet oxygen in vivo using a new fluorescent sensor, Singlet Oxygen Sensor Green. J Exp Bot. 2006;57(8):1725-34. Epub 2006 Apr 4.

图2. 肌红卵白参加脂质体后随着工夫的SOSG荧光变革(Changes in SOSG fluorescence with time after the addition of myoglobin to liposomes)。Singlet oxygen sensor green (SOSG) reagent was dissolved in methanol to make a stock solution of 500 μM. SOSG (final concentration 50 μM) was added to liposomes (2 mg ml−1) in 50 mM sodium phosphate buffer pH 7.4 in a Petri dish (2 cm diameter). Lipid oxidation was started by the addition of ferric horse myoglobin (10 μM). Following excitation of SOSG at 480 nm using a 75 W xenon lamp (Leica UK Ltd, Milton Keynes, UK) with a 480 nm interference filter and heat filter, images of the fluorescence from SOSG in the samples were produced using a Peltier-cooled (200 K) charge-coupled device (CCD) camera (576×384 pixels, Wright Instruments, Enfield, UK) with a 532 nm interference filter in the objective.

图3. 拟南芥叶子内SOSG荧光发生的动力学变革(Kinetics of the development of SOSG fluorescence in Arabidopsis leaves)。Leaves were grown at a PPFD of 200 μmol m−2 s−1, detached and infiltrated with SOSG, and then the top halves of the leaves exposed to a PPFD of 650 μmol m−2 s−1 (high light). The bottom halves were kept in the dark; images were taken after 0, 5, 10, 20, 30, and 50 min.

 

二、文献泉源:Chen T et al. Singlet Oxygen Plays an Essential Role in the Root’s Response to Osmotic Stress. Plant Physiol. 2018 Aug;177(4):1717-1727.

图4. PEG处置后单线态氧和碘化丙啶细胞浸透性的变革图(Appearance of singlet oxygen and changes in cell permeability to propidium iodide after PEG treatment)。Root tips of 7-d-old seedlings stained with propidium iodide (PI) and singlet oxygen sensor green (SOSG). Median confocal slices are shown for each of these stains, along with the corresponding bright field (BF) image. Treatments were for the times indicated on the right: no treatment; 1 to 2 h, early; 3 to 4 h, mid; 5 to 8 h, late. Zones are as follows: AM, apical meristem; BM, basal meristem; and EZ, elongation zone. The median plane of the root was imaged where opaque regions in PI staining are indicative of cell death. White outlined arrows indicate SOSG and PI staining of live cells (i.e. the stain is observed in the perimeter of the cell). Solid white arrows indicate SOSG and PI staining of dead cells (i.e. where the PI stain permeates and fills the cell). Graphs on the left show quantification of the fluorescence, calculated by estimating the area of the root (using threshold above background) in a median slice that displayed SOSG or opaque PI fluorescence (using threshold above that obtained from live cells). Scale bar, 100 µm. For quantification, error bars are se, n ≥ 8.

 

【争议点】

Singlet Oxygen Sensor Green®, SOSG不具有细胞膜浸透性,能用于细胞单线态氧(1O2)检测?

专利持无方thermo(molecular probe)官方阐明此探针不具有细胞膜浸透性,实用于溶液系统单线态氧(1O2)检测。但是,Gollmer A et al.报道SOSG能拔出活哺乳植物细胞(文献泉源:Gollmer A et al. Singlet Oxygen Sensor Green®: photochemical behavior in solution and in a mammalian cell. Photochem Photobiol. 2011 May-Jun;87(3):671-9.)。

 

订购信息:Thermo fisher原装正品,大包装拆开单卖,100μg /支,单支特价700元。ag真人生物临时现货供给,接待征询,QQ:2971634497,或德律风:###

品牌

货号

称号

规格

特价(元)

Thermo fisher

Invitrogen

S36002

Singlet Oxygen Sensor Green 单线态氧荧光探针

100μg

700

单线态氧研讨联系关系产品

Maokangbio

MX4821-1G

1,3-diphenylisobenzofuran (DPBF) 1,3-二苯基异苯并呋喃(单线态氧指示剂)

1g

320

Maokangbio

MX4822-50MG

9,10-Anthracenediyl-bis(methylene)dimalonic Acid (ABDA)  9,10-蒽二基-双(亚甲基)二丙二酸(单线态氧指示剂)

50mg

990

Maokangbio

MS4075B-1G

Rose Bengal,95% 孟加拉玫瑰红

1g

382

Maokangbio

MS5557-50MG

Chlorin e6 (Ce6)  二氢卟吩e6

50mg

972


【温馨提醒】:见ag真人ag真人生物整理的活性氧检测探针专题(Probes for reactive oxygen species)

 

相干产品:ROS研讨常用的配套产品,必要理解更多信息接待来电征询,021-54736159,QQ:2971634497。

货号

称号

规格             

代价(元)  

MX4801-1KIT

Reactive Oxygen Species (ROS) Assay Kit

活性氧(ROS)检测试剂盒

100~500T

328

MX4804-1MG

Aminophenyl fluorescein (APF) 氨基苯基荧光素

1mg

2180

MX4805-1MG

Hydroxyphenyl fluorescein (HPF) 羟苯基荧光素

1mg

2180

MX4802-50MG

H2DCFDA (DCFH-DA, DCFH) 活性氧(ROS)荧光探针           

50mg

546

M36008(Invitrogen)    

MitoSOX Red Mitochondrial Superoxide Indicator

50 µg

650

 

留意事变

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 — —Written/Edited by V. Shallan【版权归MKBioag真人一切】

 

 

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